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Evidence Guide: MSL975028 - Apply advanced embedding and microtomy skills

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975028 - Apply advanced embedding and microtomy skills

What evidence can you provide to prove your understanding of each of the following citeria?

Embed Tissue

  1. Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load
  2. Identify and prioritise specimens prior to embedding
  3. Check tissue for complete processing prior to embedding
  4. Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  5. Select appropriately sized mould
  6. Apply the use of an embedding run-sheet to check the number of pieces of tissue in each cassette and noting any discrepancies
  7. Apply procedures to prevent cross-contamination between tissues
  8. Embed tissue with embedding orientation markings
  9. Perform excess wax removal from blocks using manual scrapping and/or heat removal
  10. Inspect blocks, reject and re-embed items that do not meet quality control standards
  11. Apply quality control procedures to troubleshoot discrepancies between run-sheet and tissue embedded
  12. File and store tissue blocks to facilitate efficient retrieval as required
Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify and prioritise specimens prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check tissue for complete processing prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select appropriately sized mould

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply the use of an embedding run-sheet to check the number of pieces of tissue in each cassette and noting any discrepancies

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue with embedding orientation markings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform excess wax removal from blocks using manual scrapping and/or heat removal

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality control procedures to troubleshoot discrepancies between run-sheet and tissue embedded

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

File and store tissue blocks to facilitate efficient retrieval as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut tissue sections

  1. Check that flotation bath is ready and satisfactory for use
  2. Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch
  3. Secure block in microtome following specified safety directions
  4. Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  5. Apply microtomy techniques to trim blocks to full face without significant loss of tissue
  6. Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  7. Float ribbons onto water bath to flatten sections
  8. Pick up sections onto microscope slides ensuring patient identification on slides matches what is on block
  9. Apply procedures to prevent cross-contamination between patient tissues
  10. Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
  11. Inspect sections and reject any that do not meet quality control standards
  12. Identify, troubleshoot and resolve common section quality control issues
  13. Apply quality control procedures to troubleshoot discrepancies between run-sheet and sections on slides
Check that flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply microtomy techniques to trim blocks to full face without significant loss of tissue

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float ribbons onto water bath to flatten sections

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Pick up sections onto microscope slides ensuring patient identification on slides matches what is on block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between patient tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality control procedures to troubleshoot discrepancies between run-sheet and sections on slides

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare specialised tissues

  1. Prepare agar plugs for cytological or minute specimens
Prepare agar plugs for cytological or minute specimens

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare blood clot for processing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare friable /tiny tissue in sponges and/or wrapped to ensure tissue integrity and prevent loss during processing

  1. Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load
Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify and prioritise specimens prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check tissue for complete processing prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select appropriately sized mould

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply the use of an embedding run-sheet to check the number of pieces of tissue in each cassette and noting any discrepancies

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue with embedding orientation markings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform excess wax removal from blocks using manual scrapping and/or heat removal

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality control procedures to troubleshoot discrepancies between run-sheet and tissue embedded

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

File and store tissue blocks to facilitate efficient retrieval as required

  1. Check that flotation bath is ready and satisfactory for use
Check that flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply microtomy techniques to trim blocks to full face without significant loss of tissue

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float ribbons onto water bath to flatten sections

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Pick up sections onto microscope slides ensuring patient identification on slides matches what is on block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between patient tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality control procedures to troubleshoot discrepancies between run-sheet and sections on slides

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge